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Professor Anwesha Sarkar

Introducing Dr. Anwesha Sarkar, an accomplished Assistant Professor at Iowa State University. With a diverse academic background spanning multiple continents, her expertise lies in the fascinating realm of Single Molecule Biophysics, Atomic Force Microscopy, and Protein-protein interactions. As a vital contributor to the Nanovaccine Institute, Dr. Sarkar’s research delves into the cutting-edge world of biosensors and bioengineering, propelling advancements in the field of nanovaccines. Her journey is a testament to the power of interdisciplinary collaboration and the pursuit of scientific excellence.

Education 
  • Postdoctoral Research Associate, Iowa State University (2020) PhD
  • Physics, Wayne State University (2015) MPhil, Physics
  • Institute of Physics, India (2011) MSc,
  • Physics, IIT Madras, India (2010) BSc,
  • Physics, ST. Xavier’s College, Kolkata, India (2008)  

Email: anweshas@iastate.edu

BACM LAB – Research Laboratory of Professor Anwesha Sarkar

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Publications
  • Waite, J., Tana, S., Saha, H., Sarkar, S., Sarkar, A. (2022). Few-shot Deep Learning for AFM Force Curve Characterization of Single Molecule Interactions. Accepted in Patterns
  • Rade, J., Zhang, J., Sarkar, S., Krishnamurthy, A., Ren, J., & Sarkar, A. (2022). Deep Learning for Live Cell Shape Detection and Automated AFM Navigation. Bioengineering, 9(10), 522.
  • Sarkar, A. (December 2021). Biosensing, characterization of biosensors, and improved drug delivery approaches using Atomic Force Microscopy: A review. Frontiers in Nanotechnology
  • Sarkar, A*., Mao, S.*, Wang, Y., Song, C., LeVine, D., Wang, X., and Que, L. (2021). Microfluidic chip grafted with integrin tension sensors for evaluating the effects of flowing shear force and ROCK inhibitor on platelets. Lab on a chip, (* made equal contribution and they share the first authorship)
  • Sarkar, A., LeVine, D. N., Kuzmina, N., Zhao, Y., & Wang, X. (2020). Cell migration driven by self-generated integrin ligand gradient on ligand-labile surfaces. Current Biology, 30(20), 4022-4032. doi: https://doi.org/10.1016/j.cub.2020.08.020
  • Sarkar, A., LeVine, D., Zhao, Y., Mollaeian, K., Ren, J., & Wang, X. (2020). Tandem tension sensor reveals substrate rigidity-dependence of integrin molecular tensions in live cells. bioRxiv. doi: https://doi.org/10.1101/2020.01.24.918946
  • Zhao, Y., Sarkar, A., & Wang, X. (2020). Peptide nucleic acid based tension sensor for cellular force imaging with strong DNase resistance. Biosensors and Bioelectronics, 150, 111959. doi: https://doi.org/10.1016/j.bios.2019.111959
  • Wang, Y., Zhao, Y., Sarkar, A., & Wang, X. (2019). Optical sensor revealed abnormal nuclease spatial activity on cancer cell membrane. Journal of biophotonics, 12(5), e201800351. DOI: 10.1002/jbio.201800351
  • Sarkar, A., Zhao, Y., Wang, Y., & Wang, X. (2018). Force-activatable coating enables high-resolution cellular force imaging directly on regular cell culture surfaces. Physical biology, 15(6), 065002. doi: 10.1088/1478-3975/aac69d
  • Wang, Y., LeVine, D. N., Gannon, M., Zhao, Y., Sarkar, A., Hoch, B., & Wang, X. (2018). Force-activatable biosensor enables single platelet force mapping directly by fluorescence imaging. Biosensors and Bioelectronics, 100, 192-200. DOI: 10.1016/j.bios.2017.09.007
  • Zhao, Y., Wang, Y., Sarkar, A., & Wang, X. (2018). Keratocytes generate high integrin tension at the trailing edge to mediate rear De-adhesion during rapid cell migration. Iscience, 9, 502-512. doi: https://doi.org/10.1016/j.isci.2018.11.016
  • Sarkar, A.#, Sohail, A., Dong, J., Prunotto, M., Shinki, K., Fridman, R., & Hoffmann, P. M. (2019). Live cell measurements of interaction forces and binding kinetics between Discoidin Domain Receptor 1 (DDR1) and collagen I with atomic force microscopy. Biochimica et Biophysica Acta (BBA)-General Subjects, 1863(11), 129402. DOI: 10.1016/j.bbagen.2019.07.011
  • Jones, S. K., Sarkar, A.#, Feldmann, D. P., Hoffmann, P., & Merkel, O. M. (2017). Revisiting the value of competition assays in folate receptor-mediated drug delivery. Biomaterials, 138, 35-45. doi: https://doi.org/10.1016/j.biomaterials.2017.05.034
  • J. Rade, S. Sarkar, A. Krishnamurthy, A. Sarkar, Virtual AFM: Generating Synthetic 2D Multi-view Images from 3D Protein Structure, Biomedical Engineering Society (BMES) Annual meeting, San Antonio, TX, 2022.
  • A. Koep, A. Sarkar, S. Alam, T. Prozorov, I. Schneider, Actuatable Hollow DNA Origami Drug Delivery Constructs, Biomedical Engineering Society (BMES) Annual meeting, San Antonio, TX, 2022.
  • J. Rade, S. Sarkar, A. Sarkar, A. Krishnamurthy, 3D Reconstruction of Protein Complex Structures Using Synthesized Multi-View AFM Images, Machine Learning in Structural Biology (MLSB) Workshop at the 36th Conference on Neural Information Processing Systems (NeurIPS), New Orleans, LA, 2022.
  • A. Sarkar, N. Hassan, R. Bardhan, Multimodal characterization of live cancer cells using Atomic Force Microscopy and Raman spectroscopy, presented as a poster, Biophysical Society 66th Annual Meeting, San Francisco, CA 2022.
  • A. Sarkar, S. Yang, B. Chen, High Resolution Single Molecule Imaging of WRC-Rac1 Protein Complex with Atomic Force Microscopy, Biomedical Engineering Society (BMES) Annual meeting, Orlando, Fl 2021.
  • J. Rade, S. Sarkar, A. Krishnamurthy, J. Ren, A. Sarkar, AI Guided Measurement of Live Cells Using AFM, Modelling, Estimation and Control Conference (MECC), Austin, TX 2021.
  • A. Sarkar, J. Waite, S. Sarkar, Deep learning for fast Atomic Force Microscopy data analytics, selected for platform presentation and presented as a talk, Biophysical Society 65th Annual Meeting (virtual) 2021.
  • S. Mao~ , A. Sarkar ~, D. LeVine, X. Wang, L. Que, Studies of the drug treatment effects on platelets using integrative tension sensors embedded in a microfuidic chip, Microfluidics for Hematology, presented as talk, Chicago, IL 2020 (~ made equal contribution and share the first authorship)
  • A. Sarkar, Y. Wang, X. Wang, Determining Integrin Molecular Tension for the Recruitment and the activation of Focal Adhesion Kinase, Biophysical Society (BPS), 63rd Annual meeting, presented as poster, Baltimore, MD 2019.
  • A. Sarkar, X. Wang, Integrin molecular tensions in live cells are altered by substrate rigidity, Biophysical Society (BPS), 62nd Annual meeting, presented as a talk and poster, San Francisco, CA 2018 [the work was also presented as a talk and poster in the ISU Research Day, Iowa State University, 2018]
  • A. Sarkar, R. Fridman, A. Sohail and P. M. Hoffmann, Measurement of DDR-Collagen interaction Forces with Atomic force Microscopy, American Physical Society (APS) March Meeting, San Antonio, TX 2015
  • A. Sarkar, E. Mayyas and P. M. Hofmann, Effect of Surface Density of Active sites on Rupture Force Distributions of Single Molecule Interactions, Biophysical Society (BPS), 59th Annual Meeting, Baltimore, MD 2015
  • A. Sarkar and P. M. Hoffmann, Effect of active site density on rupture force distributions in ligand-protein force measurements, American Physical Society (APS) Ohio-Region Section Meeting, April 2014, Youngstown, OH (presented as poster) [the work was also presented during the Physics Graduate Research Day, Wayne State University, April 2014]
  • A. Sarkar, E. Kramkowski, E. Mayyas and P. M. Hoffmann, Multiple attachments, heterogeneous binding and the high force tail in protein-protein binding force histograms, American Physical Society (APS) March Meeting, March 2013, Baltimore, MD (presented as poster) [the work was also presented during the Physics Graduate Research Day, Wayne State University, April 2013]
  • A. Sarkar, E. Kramkowski and P. M. Hoffmann, Live cell imaging and determination of protein-protein rupture force with AFM, American Physical Society (APS) Ohio-Region Section Meeting, October 2012, Detroit, MI (presented as poster) [the work was also presented in Michigan Microscopy and Microanalysis Society Fall 2012 Meeting]